High sensitivity of a rat liver nucleoplasmic protein to triiodothyronine

Cyclic AMP-dependent histone-specific nucleoplasmic protein kinase from rat liver.

A nucleoplasmic histone kinase activity was isolated from livers of adult rats and purified 39-fold compared with whole nuclei by ultracentrifugation of the nuclear extract and Sephadex G-200 gel filtration in the presence of cyclic AMP. Analysis by polyacrylamide-gel electrophoresis as well as by gel filtration indicates a mol.wt. of approx. 60,000 for the catalytic subunit and 130000-150000 f...

Regulation of specific rat liver messenger ribonucleic acids by triiodothyronine.

A plasmid cDNA library was constructed using poly(A+) RNA isolated from the livers of rats treated with 3,5,3'-triiodothyronine (T3) and fed a high carbohydrate diet. This library was screened by differential colony hybridization with [32P]cDNA probes made from hypothyroid and hyperthyroid rat liver poly(A+) RNA to obtain clones representing T3-inducible mRNAs. Using plasmid cDNAs to 4 differen...

The uptake of thyroxine and triiodothyronine by rat-liver mitochondria.

The sensitivity of rat liver and yeast mitochondrial ribosomes to inhibitors of protein synthesis.

Amino acid incorporation by isolated intact rat liver mitochondria was severely inhibited by chloramphenicol, carbomycin, or sparsomycin, partially inhibited by emetine, and unatIected by erytbromycin or lincomycin. By contrast, amino acid incorporation by inner membrane-matrix fractions prepared from rat liver mitochondria by digitonin was strongly inhibited by emetine, erythromycin, and linco...

alterations of protein profile in rat liver exposed to sub-acute diazinon: a proteomics approach